12/23/2023 0 Comments Attune nxt"Press Release: Agilent to Expand Portfolio and Capabilities in Cell Analysis with Acquisition of ACEA Biosciences". "Press Release: Agilent Completes Acquisition of ACEA Biosciences". "Press Release: Agilent Introduces Breakthrough Flow Cytometer". Information in this document is subject to change without notice. "Press Release: Cytek Biosciences Receives CE Marking on Its Northern Lights (NL)-CLC Platform". Attune NxT Auto Sampler Catalog Number 4473928 Publication Number 100032905 Revision A.0 For Research Use Only. Thermo Fisher Scientific Inc., Carlsbad, CA (Life Technologies business) Attune NxT Flow Cytometer system makes multiparametric flow cytometry available to both new and experienced researchers. The Attune NxT system makes multiparametric flow cytometry available to both new and experienced researchers. Be sure to keep a time zero control as you need to know where the first generation ran.Product › Details Attune NxT Flow Cytometer After a 10–20 min incubation to undergo de-esterification, cells are ready to be set up for whatever treatment you are planning. The Invitrogen Attune NxT Flow Cytometer is an advanced cell analyzer where acoustic focusing fluids are key to both high sensitivity and high efficiency. The Invitrogen Attune NxT XE3 Computer Upgrade kit is designed for use with the Attune NxT Acoustic Focusing Cytometer. Spin down cells, wash 1x, and resuspend in complete medium. Once the dye incubation is over (20 min, 37☌), add serum or BSA (at least 1%) to scavenge any remaining unreacted dye. Gently agitate the cells during staining. Unique, powerful acoustic technology avoids clogging and dramatically reduces cell preparation and processing time even for troublesome large cells and. Add the dye to the cells and invert a few times to mix. The easiest way to do this is to make a 2x dye solution (1x = 1–10 µM) and resuspend your cells in a half volume of medium (no serum or BSA). Be sure that the cells are not sitting in a clump in the bottom of your tube. Cells are labeled rapidly, so you want to pre-dilute the dye and mix it into your cells rapidly. Thermo Fisher Attune NxT 4 lasers, 14 parameters 405, 488, 561, 640 nm lasers Plate-reading capacity (96 and 384 well) Plate auto-sampler Accepts conical. Even labeling can be achieved by starting with a uniform cell population (not a mixture of lymphocytes and granulocytes for example) as staining will be proportional to cell size. If the peak is too broad, the generations will overlap each other and the series of peaks will become a hump. This rapid acquisition rate supports the use of flow cytometry. Data was acquired on an Invitrogen Attune NxT Flow Cytometer using a 405 laser and 440/50 nm emission filter. Samples were isolated and characterized according to the method described above and quantified by flow cytometry using the Attune NxT Flow Cytometer. Statistical analysis was performed using R 4.0.3 software. In addition, total bacteria and live and dead populations can be quantified without the use of reference counting beads (Figure 2). Cells were subsequently stained by adding 2 drops of FxCycle Violet Ready Flow Reagent and incubated for 30 minutes, at 25☌. The data was analized with Attune NxT 3.2 Software. Impct and CytoCell in partnership with ThermoFischer Scientific will present to. The key to good generational profiles with CellTrace™ reagents is starting with cells that are evenly labeled so that they have a tight coefficient of variance (CV) when run at time zero after labeling. With its acoustics-assisted technology, the Attune NxT Flow Cytometer allows for fast (up to 1 mL/minute) and accurate analysis of very dilute samples. Presentation of the new Attune NxT cytometer, on January 24th.
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